Anti-phospho-H2A.X (Ser139) Antibody

Upstate®, from rabbit

H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac

fonte biológica


Nível de qualidade


forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies



species reactivity

human, vertebrates


antibody small pack of 25 μg




ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable



nº de adesão NCBI

nº de adesão UniProt

enviado em


Gene Information

Categorias relacionadas

Descrição geral

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.


Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.


Epitope: a.a. 134-142


A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.


Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.


15 kDa


Replaces: MABE205

forma física

Format: Purified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Nota de análise

Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificado de análise

Certificado de origem

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