07-164

Sigma-Aldrich

Anti-phospho-H2A.X (Ser139) Antibody

Upstate®, from rabbit

Sinônimo(s):
H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

100

forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

embalagem

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

aplicação(ões)

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

enviado em

ambient

Gene Information

Categorias relacionadas

Descrição geral

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Especificidade

Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.

Imunogênio

Epitope: a.a. 134-142

Aplicação

Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.

Qualidade

Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.

Descrição-alvo

15 kDa

Ligação

Replaces: MABE205

forma física

Format: Purified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Nota de análise

Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificado de análise

Certificado de origem

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