1.50620

Purospher^® STAR RP-18 endcapped (5µm) Hibar^® RT 250-3

suitable for HPLC

Properties

• material: stainless steel column
• Nível de qualidade: 100
• Agency: suitable for USP L1
• linha de produto: Purospher^®
• característica: endcapped
• embalagem: 1 ea of
• extensão da rotulagem: 17% Carbon loading
• parâmetro: 400 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• coluna C × D.I.: 25 cm × 3 mm
• matriz: fully porous particle
• Grupo ativo da matriz: C18 bonding phase
• tamanho de partícula: 5 μm
• tamanho de poro: 120 Å pore size
• área da superfície: 330 m²/g
• operating pH range: 1.5-10.5
• técnica de separação: reversed phase; supercritical fluid (SFC)
• temperatura de armazenamento: no temp limit

Description

Descrição geral

Purospher^® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B).
These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher^® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.

Purospher^® STAR RP-18 endcapped, 5 µm Hibar^® RT 250-3 is a ready to use stainless steel column suitable for connection to any HPLC system.
The column dimension of 250 mm length x 3 mm I.D. is the optimal choice for the high resolution HPLC separation of complex samples , allowing significant solvent savings and improved sensitivity.
The recommended guard column is Purospher^® STAR RP-18 endcapped (5 µm) LiChroCART^® 4-4 part number 1.50250.0001.
For direct connection to a Hibar^® RT columns the Precolumn holder for 4-4 LiChroCART^® cartridges, part number 1.51487.0001 can be used.

Learn more about Purospher^® STAR HPLC and UHPLC Columns

Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Aplicação

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Características e benefícios

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Nota de análise

Pressure: ≤ 120 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 3.0

Informações legais

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany