Sensitive, specific detection of His•Tag fusion proteins
The HIS TAG® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the HIS TAG® sequence encoded by many of the NOVAGEN® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of HIS TAG® fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG® Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG® sequences. This antibody also detects the recombinant marker bands in the Perfect Protein<TMSYMBOL></TMSYMBOL> and Trail Mix<TMSYMBOL></TMSYMBOL> Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.
* Not recommended for HRP-based detection of Trail Mix Western Markers.
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Toxicity: Standard Handling (A)
Informações legais
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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The Plant journal : for cell and molecular biology, 112(6), 1462-1472 (2022-11-12)
Histone deacetylases (HDAs) regulate many aspects of plant development and responses to environmental changes. Previous studies have demonstrated that the Arabidopsis histone deacetylase HDA15 is a positive regulator in far-red (FR) light-mediated inhibition of hypocotyl elongation. Furthermore, HDA15 can be
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