ABS1507

Anti-Aurora B

from rabbit, purified by affinity chromatography

• Sinônimo(s): Aurora kinase B, EC:2.7.11.1, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): mouse (based on 100% sequence homology)
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_001243763.1
• nº de adesão UniProt: Q96GD4
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... AURKB(9212) ; mouse ... Aurkb(20877)

Descrição

Descrição geral

Aurora kinase B (UniProt: Q96GD4; also known as EC:2.7.11.1, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B) is encoded by the AURKB (also known as AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5) gene (Gene ID: 9212) in human. Aurora Kinase B is a serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. It is a key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Aurora kinase B phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP and phosphorylation of INCENP leads to increased Aurora kinase B activity. Five isoforms of Aurora Kinase are reported that are produced by alternative splicing. Its expression is shown to be cell-cycle regulated, with low expression in G1/S phase and an increase during G2 and M phase. Following M phase its expression declines. Cancer cells display high expression of Aurora kinase B during M phase. Disruption in regulation of Aurora kinase B causes perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. High level expression is observed in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Substitution of Lysine 106 with arginine leads to loss of its kinase activity and severely impairs mitotic progression.

Especificidade

This rabbit polyclonal antibdy detects Aurora kinase B in human cells.

Imunogênio

Epitope: C-terminus

KLH-conjugated Linear Peptide corresponding to the 11 amino acids from the C-terminal region of Human Aurora kinase B.

Aplicação

Anti-Aurora B, Cat. No. ABS1507, is a rabbit polyclonal antibody that detects Aurora Kinase B and has been tested for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Aurora B in HeLa cells.

Research Category
Signaling

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Aurora B in 10 µg of HeLa cell lysate.

Descrição-alvo

~39 kDa observed; 39.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable