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MABT205

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A

clone DM1A, from mouse

Sinônimo(s):

Tubulin alpha-1 chain

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

DM1A, monoclonal

reatividade de espécies

mouse, human

reatividade da espécie (prevista por homologia)

chicken (based on 100% sequence homology)

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... TUBA1A(7846)

Descrição geral

Tubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.

Imunogênio

Native chicken brain microtubules.

Aplicação

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.

Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.

Qualidade

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.

Descrição-alvo

~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.).

Ligação

Replaces: 04-1117

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HeLa cell lysate.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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