10108090001

Peroxidase (POD)

grade I, from horseradish

• Synonym(s): POD, peroxidase
• Enzyme Commission number: 1.11.1.7 (BRENDA, IUBMB)

Properties

• fonte biológica: horseradish
• Nível de qualidade: 100
• forma: lyophilized (salt-free)
• specific activity: ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.); 250 U/mg
• embalagem: pkg of 25,000 U
• manufacturer/tradename: Roche
• pH ideal: 6.0-6.5
• enviado em: wet ice
• temperatura de armazenamento: 2-8°C

Description

Descrição geral

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Aplicação

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

Embalagem

25000 units

Ações bioquímicas/fisiológicas

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

Qualidade

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A₄₀₃/A₂₇₅)

Nota de preparo

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Reconstituição

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317 - H334
• Declarações de precaução: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable