Merck
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11442074001

Roche

BM-Purple

Roche, pkg of 100 mL, solution

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Sinônimo(s):
chromogenic substrate

forma

solution

Nível de qualidade

100

embalagem

pkg of 100 mL

manufacturer/tradename

Roche

cor

dark purple

solubilidade

H2O: insoluble

enviado em

wet ice

temperatura de armazenamento

2-8°C

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Este Item
870865P860856PB3528
BM-Purple Roche, pkg of 100 mL, solution

Roche

11442074001

BM-Purple

BMP22 Avanti Polar Lipids, powder

Avanti

870865P

BMP22

RBM14C16 Avanti Polar Lipids, powder

Avanti

860856P

RBM14C16

Sigma-Aldrich

Sigma-Aldrich

B3528

BSK-H Medium

packaging

pkg of 100 mL

packaging

pkg of 1 × 1 mg (870865P-1mg), pkg of 1 × 5 mg (870865P-5mg)

packaging

package of 1 × 1 mg

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Avanti Polar Lipids

manufacturer/tradename

Avanti Polar Lipids

manufacturer/tradename

-

color

dark purple

color

-

color

-

color

-

solubility

H2O: insoluble

solubility

-

solubility

-

solubility

-

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Descrição geral

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

Aplicação

  • Colorimetric AP substrate for: Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

Componentes

Ready-to-use solution.

Nota de preparo

Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

does not flash

Ponto de fulgor (ºC)

does not flash

Certificates of Analysis (COA)

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Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (Exemplo T1503).

Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

insira como1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Produtos Aldrich

  • Se encontrar um número de lote como TO09019TO - insira o número do lote 09019TO sem as duas primeiras letras.

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DIG RNA Labeling Kit (SP6/T7) sufficient for 2 x 10 labeling reactions, kit of 1 (12 components), suitable for hybridization, suitable for Southern blotting

Roche

11175025910

DIG RNA Labeling Kit (SP6/T7)

BCIP®/NBT solution, premixed

Supelco

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BCIP®/NBT solution, premixed

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Roche

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BCIP

NBT-BCIP® solution BioReagent, suitable as substrate for alkaline phosphatase in dot blots

Sigma-Aldrich

72091

NBT-BCIP® solution

NBT 4-Nitro blue tetrazolium chloride, solution

Roche

11383213001

NBT

Fluorescein RNA Labeling Mix solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

Roche

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Fluorescein RNA Labeling Mix

Bacteriorhodopsin from Halobacterium salinarum native sequence, lyophilized powder

Sigma-Aldrich

B0184

Bacteriorhodopsin from Halobacterium salinarum

Christoph Kessle
Nonradioactive Analysis of Biomolecules null
Valentina Garcia-Lee et al.
The International journal of developmental biology, 65(4-5-6), 395-401 (2020-09-16)
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Precise cis-regulatory control of gene expression is essential for normal embryogenesis and tissue development. The BMP antagonist Gremlin1 (Grem1) is a key node in the signalling system that coordinately controls limb bud development. Here, we use mouse reverse genetics to
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Osteoblast differentiation is sequentially characterized by high rates of proliferation followed by increased protein and matrix synthesis, processes that require substantial amino acid acquisition and production. How osteoblasts obtain or maintain intracellular amino acid production is poorly understood. Here, we
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