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11681451001

Roche

NBT/BCIP Stock Solution

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Sinônimo(s):
5-bromo-4-chloro-3-indoyl phosphate, BCIP

forma

solution

Nível de qualidade

100

peso molecular

817.7

embalagem

pkg of 8 mL

manufacturer/tradename

Roche

temperatura de armazenamento

2-8°C

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Este Item
116814600011138322100111383213001
NBT/BCIP Stock Solution

Roche

11681451001

NBT/BCIP Stock Solution

INT/BCIP Stock Solution solution, pkg of 3 mL

Roche

11681460001

INT/BCIP Stock Solution

BCIP solution, >95% (HPLC), pkg of 3 mL (150 mg)

Roche

11383221001

BCIP

NBT 4-Nitro blue tetrazolium chloride, solution

Roche

11383213001

NBT

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

mol wt

817.7

mol wt

(INT: Mr = 505.7; BCIP toluidine salt: Mr = 433.6)

mol wt

-

mol wt

Mr 817.7

packaging

pkg of 8 mL

packaging

pkg of 3 mL

packaging

pkg of 3 mL (150 mg)

packaging

pkg of 3 mL (300 mg)

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

Descrição geral

The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

Evaluation: visually

Aplicação

  • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque lifts
  • In situ hybridization
  • and for applications in immunohistochemistry and immunocytochemistry.
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

forma física

Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.

Nota de preparo

Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
  • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Note: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

does not flash

Ponto de fulgor (ºC)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (Exemplo T1503).

Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

insira como1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Produtos Aldrich

  • Se encontrar um número de lote como TO09019TO - insira o número do lote 09019TO sem as duas primeiras letras.

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Blocking Reagent For nucleic acid hybridization and detection

Roche

11096176001

Blocking Reagent

BCIP®/NBT-Blue Liquid Substrate System for Membranes alkaline phosphatase substrate

Sigma-Aldrich

B3804

BCIP®/NBT-Blue Liquid Substrate System for Membranes

BCIP®/NBT-Purple Liquid Substrate System for Membranes ready to use alkaline phosphatase substrate

Sigma-Aldrich

B3679

BCIP®/NBT-Purple Liquid Substrate System for Membranes

DIG RNA Labeling Mix sufficient for 20 reactions, solution

Roche

11277073910

DIG RNA Labeling Mix

Streptavidin-AP conjugate solution, pkg of 1 mL (1,000U)

Roche

11089161001

Streptavidin-AP conjugate

NBT crystals, pkg of 5 g

Roche

11585029001

NBT

DIG Wash and Block Buffer Set storage temp.:2-8°C

Roche

11585762001

DIG Wash and Block Buffer Set

DIG RNA Labeling Kit (SP6/T7) sufficient for 2 x 10 labeling reactions, kit of 1 (12 components), suitable for hybridization, suitable for Southern blotting

Roche

11175025910

DIG RNA Labeling Kit (SP6/T7)

DIG Nucleic Acid Detection Kit sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting

Roche

11175041910

DIG Nucleic Acid Detection Kit

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