Merck
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G418-RO

Roche

G-418 Solution

solution, =98% (TLC), suitable for transfection

Sinônimo(s):
G 418 disulfate salt solution, O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt, Antibiotic G418, neomycin
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
Número CAS:
Peso molecular:
692.71
Número MDL:
ID de substância PubChem:

Nível de qualidade

100

teor

=98% (TLC)

forma

solution

peso molecular

692.7 Da

embalagem

pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g])
pkg of 20 mL (04727878001 [equivalent to 1 g])

manufacturer/tradename

Roche

concentração

50 mg/mL

technique(s)

transfection: suitable

espectro de atividade do antibiótico

protozoa

Modo de ação

protein synthesis | inhibits

enviado em

wet ice

temperatura de armazenamento

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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Descrição geral

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

Aplicação

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neor) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Ações bioquímicas/fisiológicas

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Características e benefícios

  • Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
  • Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
  • Function tested in a cell culture assay - ensures effectiveness.
  • Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
  • Cost effective, since the selected colonies are stable.
Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Qualidade

Purity: = 98%, as shown by TLC.
Function testing: in cell culture.

Nota de preparo

Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

Outras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

No data available

Ponto de fulgor (ºC)

No data available

Certificado de análise

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Certificado de origem

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Product Information Sheet

Mais documentos

Quotes and Ordering

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Artigos

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

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