Merck
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17779

Millipore

2,3,5-Triphenyl-tetrazolium chloride solution

suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Synonym(s):
TTC solution, TTC supplement, TTC solution, TTC supplement
Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
Molecular Weight:
334.80
Beilstein:
3923356
Número MDL:
ID de substância PubChem:
NACRES:
NA.85

Nível de qualidade

100

esterilidade

sterile (Filtered and Aseptic Handled)

forma

liquid

prazo de validade

limited shelf life, expiry date on the label

peso molecular

334.8 g/mol

application(s)

agriculture
environmental
food and beverages

microbiology

temperatura de armazenamento

2-8°C

adequação

bacteria

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

Descrição geral

2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.

Aplicação

2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media

Componentes

(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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