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39465

Millipore

Gelatina

suitable for microbiology, ultrahigh gel strength

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
41106212
NACRES:
NA.85

fonte biológica

Porcine

Nível de qualidade

forma

powder

qualidade

ultrahigh gel strength

prazo de validade

limited shelf life, expiry date on the label

perda

9.5-12.5% loss on drying

transmitância

450 nm, ≥85%
620 nm, ≥95%

pH

5.20-5.60

condutividade

120-190 μS/cm at 30 °C (1%)

viscosidade

5.10-5.80 mPa.s

força do gel

280-302 g BloomAOAC

solubilidade

H2O: 67 mg/mL at 60 °C

traços de cátion

Ca: ≤100 mg/kg

aplicação(ões)

microbiology

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Aplicação

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Componentes

A gelatina é uma mistura heterogênea de proteínas solúveis em água com massas moleculares médias altas, presente no colágeno. As proteínas são extraídas fervendo a pele, tendões, ligamentos, ossos etc. relevantes em água. A gelatina tipo A é derivada de tecido curado com ácido. A tipo B é derivado de tecido curado com limão.

Atenção

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota de preparo

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Johno Breeze et al.
Journal of the Royal Army Medical Corps, 159(2), 84-89 (2013-05-31)
Ballistic gelatin is the most common tissue simulant used to reproduce the penetration of projectiles into muscle but published data to support its use are primarily based on bullets, despite explosive fragments being the most common cause of injury to
M V Nasekin et al.
Anesteziologiia i reanimatologiia, (1)(1), 59-60 (2013-07-03)
The article deals with skills improving problem of epidural anaesthesia with ultrasound control. Methods of gelatin spinal column model making, use and its economical side are discussed in the article.
Ivy Kanungo et al.
Materials science & engineering. C, Materials for biological applications, 33(4), 2455-2459 (2013-03-19)
The dielectric behavior of the gelatine-GAGs based blend systems has been studied to understand the dynamic behavior of the water at the protein-GAGs interfaces which are relevant for tissue engineering application. Impedance (Z) and phase have been measured as a
Delia Mihaela Iurea et al.
Journal of nanoscience and nanotechnology, 13(6), 3841-3850 (2013-07-19)
New sub-micronic capsules based on a copolymer of maleic anhydride-alt-vinyl acetate and a natural polymer (gelatin) using an interfacial condensation method were obtained. Sub-micronic capsules were characterized by Fourier Transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) method, zeta-potential, scanning
Ashley R Flory et al.
BMC biotechnology, 13, 28-28 (2013-03-28)
It is important for industries to find green chemistries for manufacturing their products that have utility, are cost-effective and that protect the environment. The paper industry is no exception. Renewable resources derived from plant components could be an excellent substitute

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