Merck
All Photos(1)

60983

Millipore

Kovac′s reagent for indoles

suitable for microbiology

Synonym(s):
4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac
Empirical Formula (Hill Notation):
C9H11NO
CAS Number:
Molecular Weight:
149.19
Beilstein:
4132845
Número MDL:
ID de substância PubChem:
NACRES:
NA.85

prazo de validade

limited shelf life, expiry date on the label

Nível de qualidade

100

composição

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

technique(s)

microbe id | specific enzyme detection: suitable

índice de refração

n20/D 1.417

application(s)

agriculture
clinical testing
environmental
food and beverages

microbiology

temperatura de armazenamento

2-8°C

adequação

Escherichia coli
coliforms

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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Descrição geral

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Aplicação

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Ações bioquímicas/fisiológicas

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

3 - Flammable liquids

WGK

WGK 1

Ponto de fulgor (ºF)

96.8 °F

Ponto de fulgor (ºC)

36 °C

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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