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A7906

Albumina sérica bovina

heat shock fraction, pH 7, ≥98%

Sinônimo(s):

Albumina bovina, Albumina, soro bovino, BSA

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Para você/SKUDisponibilidadePreço
50 g

Disponível para envio HOJEdeCajamar

R$ 931,00
100 g

Disponível para envio HOJEdeCajamar

R$ 1.449,00
500 g

Disponível para envio HOJEdeCajamar

R$ 6.268,00
1 kg

Disponível para envio HOJEdeCajamar

R$ 9.693,00

Sobre este item

Número CAS:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
blood typing: suitable, microbiological culture: suitable
Impurities:
≤5.0% Loss on drying

R$ 931,00


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biological source

bovine

Quality Segment

assay

≥98%

form

lyophilized powder

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blood typing: suitable, microbiological culture: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Application

A albumina sérica bovina foi usada -
  • como um componente de mistura para PCR (reação em cadeia da polimerase)
  • no preparo de neurônios corticais dissociados
  • durante marcação imunofluorescente
  • como um componente de solução salina isenta de Ca2+ (contendo colagenase, hialuronidase e DNAse) usada para digestão de tecido bovino

Biochem/physiol Actions

Certos epítopos conformacionais e sequência primária de BSA são alérgenos suspeitos em alergias a carne de vaca e leite em humanos.

Preparation Note

Pode-se referir à albumina sérica como Fração V. Esta convenção de nomenclatura é derivada do método original de Cohn para fracionamento de proteínas séricas usando precipitação com etanol frio. A albumina sérica foi encontrada na quinta fração de etanol usando o método de Cohn. Desde então, o termo “Fração V” foi usado por alguns para descrever a albumina sérica, independentemente do método de preparo. Outros usaram este termo para descrever a albumina sérica purificada por métodos de fracionamento com etanol que foram altamente modificados desde o método de Cohn original foi descrito. A Sigma-Aldrich fabrica e distribui albuminas séricas purificadas a partir de diversos métodos primários, inclusive o método de fracionamento de Cohn verdadeiro, métodos de fracionamento de etanol modificados, choque térmico e cromatografia. Etapas de purificação adicionais podem incluir cristalização ou filtração de carvão.
Preparado usando fracionamento por choque térmico

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Este Item
A8022A9543B4287
description

heat shock fraction, pH 7, ≥98%

description

heat shock fraction, pH 5.2, ≥96%

description

heat shock fraction, low endotoxin, pH 7, ≥98%

description

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

technique(s)

blood typing: suitable, microbiological culture: suitable

technique(s)

ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable

technique(s)

ELISA: suitable, electrophoresis: suitable, western blot: suitable

technique(s)

ELISA: suitable, electrophoresis: suitable, western blot: suitable

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

assay

≥98%

assay

≥96%

assay

≥98%

assay

≥98%

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder


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Classe de armazenamento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–10 of 10 Questions  
  1. Need the exact molecular weight and elemental formula or amino acid sequence for this product.

    1 answer
    1. Bovine Serum Albumin (BSA) has an approximate molecular weight of 66 kDa. Please see the link below to review the BSA sequence information published at UniProt:
      https://www.uniprot.org/uniprotkb/P02769/entry#sequences

      Additional information is available in the product datasheet provided at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/237/a7906pis.pdf

      Helpful?

  2. How long is BSA (A7906) viable at room temperature? 200g was left on benchtop overnight (Approximately 22 hours), will it still work?

    1 answer
    1. This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
      Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.

      Helpful?

  3. Is the product A7906, Albumin from bovine serum (BSA), fatty acid-free?

    1 answer
    1. Helpful?

  4. After reconstitution in PBS, for how long is the protein stable in solution if stored at 4°C or -20*C?

    1 answer
    1. Prepared BSA solution products also available for purchase. These products have a shelf life ranging from 18 months to 2 years. Many are assigned a restest schedule with no firm expiration date. These products are prepared in saline, DPBS, or Tris. These solutions are filtered products and offered in a range of concentrations. The storage recommendation for these products is 2 - 8 deg. C. In addition, BSA is often used as a carrier protein for less stable compounds that are stored frozen. While there is no established shelf life for a BSA solution stored frozen, it is quite stable. See the link below for a sample Certificate of Analysis for one of the BSA solutions.

      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A9576-50MLSLCL8813.pdf

      Helpful?

  5. Does this BSA have bovine immunoglobulins?

    1 answer
    1. This product does contain bovine immunoglobulins. BSA products that do not contain immunoglobulins will state "globulin-free" or "essentially globulin-free" on the product detail page. Product A3156 is an example of an immunoglobulin free BSA product.

      Helpful?

  6. Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?

    1 answer
    1. We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. Is Product A7906, Albumin from bovine serum (BSA), protease-free?

    1 answer
    1. We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.

      Helpful?

  9. Is Product A7906, Albumin from bovine serum (BSA), suitable for use as a blocking agent in ELISA?

    1 answer
    1. We have not tested the use of A7906 in ELISA. Because the remainder of the product is mostly globulins, we would not suggest A7906 for ELISA use.

      Helpful?

  10. Is Product A7906, Albumin from bovine serum (BSA), tested for insulin levels?

    1 answer
    1. A7906 is not tested in quality control for insulin levels. One BSA that is indicated as "suitable for insulin RIA procedures" is A7888.

      Helpful?

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