Todas as fotos(7)

D0632

Sigma-Aldrich

DL-Dithiothreitol

≥98% (HPLC), ≥99.0% (titration)

Sinônimo(s):
threo-1,4-Dimercapto-2,3-butanediol, DTT, Cleland’s reagent
Fórmula linear:
HSCH2CH(OH)CH(OH)CH2SH
Número CAS:
Peso molecular:
154.25
Beilstein:
1719757
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.21

Nível de qualidade

200

teor

≥98% (HPLC)
≥99.0% (titration)

reaction suitability

reagent type: reductant

pf

41-44 °C (lit.)

solubilidade

H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow

temperatura de armazenamento

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Descrição geral

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Aplicação

DTT has been used:
  • as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.
  • as a component of medium for the demembranation and reactivation of spermatozoa.
  • to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.
  • as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Embalagem

1 kg in poly drum
1, 5, 10, 25, 100 g in poly bottle

Pictogramas

CorrosionExclamation mark

Palavra indicadora

Danger

Frases de perigo

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK Alemanha

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de análise

Certificado de origem

Daniel C Marcus et al.
Auditory neuroscience, 1, 101-109 (1994-01-01)
Vestibular dark cell epithelium was isolated from the semicircular canal of gerbils to test the proposal that the sulfhydryl alkylating agent N-ethylmaleimide (NEM) inhibits K(+) secretion by this tissue and does so by reacting with a site in or near...
S Elsayed et al.
Scandinavian journal of immunology, 60(5), 486-493 (2004-11-16)
Alphas1-Casein (CAS1_BOVIN), the major allergen of cow's milk (CM), is widely used as hydrolysates in infant diet formulae and additive to other processed food items. To date, most of the reported B-cell epitope mapping were performed on polyethylene pins or...
O Linhart et al.
Reproduction (Cambridge, England), 124(5), 713-719 (2002-11-06)
This study investigated the effects of different environmental conditions on the motility parameters of paddlefish (Polyodon spathula) spermatozoa. Paddlefish spermatozoa demonstrated the following characteristics: (i) all spermatozoa were motile 10 s after activation with a velocity of 130-160 microm s(-1);...
R Quiles et al.
Clinical chemistry, 39(3), 500-503 (1993-03-01)
An automated method based on the principles of flow-injection analysis is proposed for the enzymatic determination of sodium ion in serum. The method relies on the activation of beta-galactosidase by the analyte. The features of the proposed method include linear...
Florian Füssl et al.
mAbs, 11(1), 116-128 (2018-10-09)
Charge variant analysis is a widely used tool to monitor changes in product quality during the manufacturing process of monoclonal antibodies (mAbs). Although it is a powerful technique for revealing mAb heterogeneity, an unexpected outcome, for example the appearance of...

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