Merck
Todas as fotos(4)

I5502

Sigma-Aldrich

Isopropyl β-D-1-thiogalactopyranoside

≥99% (TLC)

Sinônimo(s):
Isopropyl β-D-thiogalactoside, IPTG
Empirical Formula (Hill Notation):
C9H18O5S
Número CAS:
Peso molecular:
238.30
Beilstein:
4631
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.31

Nível de qualidade

300

descrição

non-ionic

teor

≥99% (TLC)

forma

powder

technique(s)

thin layer chromatography (TLC): suitable

Impurezas

10.0-22.0% Dioxane

temperatura de armazenamento

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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Embalagem

1, 5, 10 g in poly bottle

Aplicação

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Outras notas

Non-metabolizable galactose analog.

Reconstituição

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Pictogramas

Exclamation markHealth hazard

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Carc. 2 - Eye Irrit. 2

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

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Certificado de origem

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