Todas as fotos(4)
I5502
Isopropyl β-D-1-thiogalactopyranoside
≥99% (TLC)
Sinônimo(s):
Isopropyl β-D-thiogalactoside, IPTG
Empirical Formula (Hill Notation):
C9H18O5S
Número CAS:
Peso molecular:
238.30
Beilstein:
4631
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.31
Nível de qualidade
300
descrição
non-ionic
teor
≥99% (TLC)
forma
powder
technique(s)
thin layer chromatography (TLC): suitable
Impurezas
10.0-22.0% Dioxane
temperatura de armazenamento
2-8°C
SMILES string
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
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Categorias relacionadas
Embalagem
1, 5, 10 g in poly bottle
Aplicação
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Outras notas
Non-metabolizable galactose analog.
Reconstituição
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Carc. 2 - Eye Irrit. 2
Código de classe de armazenamento
11 - Combustible Solids
WGK
WGK 3
Ponto de fulgor (ºF)
Not applicable
Ponto de fulgor (ºC)
Not applicable
Certificado de análise
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Certificado de origem
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Cotações e pedidos
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
Therese Wohlschlager et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(27), E2787-E2796 (2014-06-01)
Effector proteins of innate immune systems recognize specific non-self epitopes. Tectonins are a family of β-propeller lectins conserved from bacteria to mammals that have been shown to bind bacterial lipopolysaccharide (LPS). We present experimental evidence that two Tectonins of fungal
Simon van Vliet et al.
BMC microbiology, 14, 116-116 (2014-06-03)
Bacterial habitats, such as soil and the gut, are structured at the micrometer scale. Important aspects of microbial life in such spatial ecosystems are migration and colonization. Here we explore the colonization of a structured ecosystem by two neutrally labeled
B K Kishore Reddy et al.
Antimicrobial agents and chemotherapy, 58(6), 3312-3326 (2014-04-02)
Pantothenate kinase (PanK) catalyzes the phosphorylation of pantothenate, the first committed and rate-limiting step toward coenzyme A (CoA) biosynthesis. In our earlier reports, we had established that the type I isoform encoded by the coaA gene is an essential pantothenate
Farshid Hadifar et al.
PloS one, 9(10), e108420-e108420 (2014-10-21)
Killed avian influenza virus (AIV) vaccines have been used to control H5N1 infections in countries where the virus is endemic. Distinguishing vaccinated from naturally infected birds (DIVA) in such situations however, has become a major challenge. Recently, we introduced the
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