Merck
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I6758

Sigma-Aldrich

IPTG

≥99% (TLC), ≤0.1% Dioxane

Synonym(s):
Isopropyl β-D-thiogalactoside, Isopropyl β-D-1-thiogalactopyranoside, IPTG
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
Beilstein:
4631
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.31

Nível de qualidade

300

fonte biológica

synthetic

descrição

non-ionic

teor

≥99% (TLC)

forma

powder

technique(s)

thin layer chromatography (TLC): suitable

Impurezas

≤0.1% Dioxane

temperatura de armazenamento

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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Embalagem

1, 5, 10 g in glass bottle

Aplicação

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Outras notas

Non-metabolizable galactose analog.

Reconstituição

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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