This product may be stored at room temperature.
P1379
TWEEN® 20
viscous liquid
Sinônimo(s):
Monolaurato de polietilenoglicol sorbitano, Monolaurato de polioxietileno sorbitano
About This Item
Produtos recomendados
descrição
non-ionic
Nível de qualidade
Ensaio
>40% (GC)
Formulário
viscous liquid
peso molecular
~1228
composição
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentração
≥40.0% (GC)
técnica(s)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
Impurezas
≤3.0% water
índice de refração
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
número de acidez
≤2.2 mg/g
índice de hidroxila
96‑108 mg/g
temperatura de transição
cloud point 76 °C
densidade
1.095 g/mL at 25 °C (lit.)
HLB
16.7
adequação
complies for IR spectroscopy
cadeia de caracteres SMILES
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
chave InChI
HMFKFHLTUCJZJO-UHFFFAOYSA-N
Procurando produtos similares? Visita Guia de comparação de produtos
Descrição geral
Os surfactantes, que possuem componentes hidrofílicos e hidrofóbicos, dividem-se em duas categorias principais: iônicos, que se ionizam na água (aniônicos, catiônicos ou anfolíticos), e não iônicos, que não se ionizam. O produto discutido é um surfactante não iônico com diversas aplicações, como emulsificação, dispersão, solubilização e uso em detergentes. Além disso, ele pode servir como um solubilizador para proteínas de membrana.
Aplicação
O TWEEN 20 foi usado como agente bloqueador para imunoensaios à base de membrana em uma concentração típica de 0,05%. O TWEEN 20 pode ser usado para a lise de células de mamíferos em uma concentração de 0,005% a 0,5%.
Características e benefícios
- Surfactante altamente versátil para suas pesquisas em bioquímica e ciências da vida
- Excelente agente solubilizante, dispersante e emulsionante
- Adequado para extração de DNA e RNA, cultura de células de mamíferos e purificação de proteínas
Embalagem
Cada kit contém 3 amostras de 100 ml, sendo cada amostra de um lote fabricado de forma única.
Nota de preparo
Outras notas
Informações legais
geralmente comprado junto com este produto
produto comparável
recomendado
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
527.0 °F - Pensky-Martens closed cup
Ponto de fulgor (°C)
275 °C - Pensky-Martens closed cup
Equipamento de proteção individual
Eyeshields, Gloves
Escolha uma das versões mais recentes:
Certificados de análise (COA)
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Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
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Artigos
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Protocolos
This page shows how to solubilize membrane proteins with products from Cytiva.
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What is the storage temperature of Tween 20?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the minimum dilution ratio of Tween20 in Exosome incubating buffer based on CMC level?
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This product is not tested for any specific application. The critical micelle concentration of TWEEN 20 has been reported as 60 milligrams per liter. Please see the link below to review a publication that may be helpful:
https://open.clemson.edu/cgi/viewcontent.cgi?article=5274&context=all_thesesHelpful?
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Please provide the information on product container and material for P1379-500ML TWEEN® 20
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This item is packaged in a 500 ML HDPE Bottle with a red PP cap w/ PTFE liner.
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You state 0.06 mM as the CMC for this product. What is the stock concentration (in Molar) for this product? Alternatively what is the % to reach that 0.06 mM / CMC concentration? Thank you
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The critical micelle concentration (CMC) reported on the product page is a value reported from the literature. This product is a neat or pure liquid. The base molecular weight of this material is 1,225 daltons and the density is 1.095 g/mL. However, this is based on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid. Please see the link below to review the general Tween 20 datasheet, note that the CMC value listed is 60 mg/L.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/128/958/p9416pis.pdfHelpful?
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What is the grade/agency of Tween 20?
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This product is a research grade TWEEN 20.
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What is the expiry date once opened?
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Long-term stability studies have not been performed, however, providing that the product is stored and handled properly, the expected shelf life is not expected to change. Factors such as improper closure and cross-contamination would certainly impact the overall stability and shelf life.
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The data sheet for my lot says this is 50% pure. Does that mean it is 50% tween 20?
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The 50% is referring to the concentration of lauric acid which occurs in the highest concentration. Fatty acid constituents of this product are determined by transesterification to yield fatty acid methyl esters, which are identified by gas chromatography. Fatty acids that are typically present in the offered Tween products are not a quantitative measurement. These include myristic, palmitic, and stearic acid, for the most part. While the Tween is considered a 'pure' product, the molecular weight is calculated on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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