Merck
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P1379

Sigma-Aldrich

TWEEN® 20

viscous liquid

Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
Número MDL:
NACRES:
NA.21

descrição

non-ionic

Nível de qualidade

300

teor

≥40.0% (GC)

forma

viscous liquid

peso molecular

~1228

composição

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

technique(s)

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

Impurezas

≤3.0% water

índice de refração

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

número de acidez

≤2.2 mg/g

índice de hidroxila

96‑108 mg/g

temperatura de transição

cloud point 76 °C

densidade

1.095 g/mL at 25 °C (lit.)

HLB

16.7

adequação

complies for IR spectroscopy

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Descrição geral

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Aplicação

TWEEN 20 has been used as a component of:
  • washing buffer and blocking buffer in Western blotting
  • blocking buffer in immunohistochemistry
  • washing buffer in ELISA (enzyme linked immunosorbent assay)
  • reaction mixture in PCR (polymerase chain reaction)
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Non-ionic detergent

Embalagem

25, 100, 250, 500, 6×500 mL in poly bottle
1, 4 L in poly bottle
P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.

Nota de preparo

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Informações legais

TWEEN is a registered trademark of Croda International PLC

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

527.0 °F - Pensky-Martens closed cup

Ponto de fulgor (ºC)

275 °C - Pensky-Martens closed cup

Equipamento de proteção individual

Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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