Merck
All Photos(1)

P8341

Sigma-Aldrich

TWEEN® 20

Low-peroxide; Low-carbonyls

Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
Número MDL:
NACRES:
NA.21

Nível de qualidade

200

descrição

non-ionic

forma

viscous liquid

peso molecular

~1228

Impurezas

≤0.5 μmol/g Peroxides
≤1.0 μmol/g Carbonyls
≤3.0% water

índice de refração

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

temperatura de transição

cloud point 76 °C

densidade

1.095 g/mL at 25 °C (lit.)
1.1 g/cm3 at 25 °C (lit.)

HLB

16.7

temperatura de armazenamento

−20°C

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Descrição geral

Tween®20, is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]

Aplicação

TWEEN 20 is widely used in several biochemical applications. It has been used:
  • As an emulsifying agent for the preparation of stable oil-in-water emulsions[2]
  • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)[3]
  • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
  • For lysing mammalian cells at a concentration of 0.005 to 0.5%
Non-ionic detergent

Embalagem

10, 5×10 mL in glass bottle

Outras notas

Preservative Free

Informações legais

TWEEN is a registered trademark of Croda International PLC

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

527.0 °F - Pensky-Martens closed cup

Ponto de fulgor (ºC)

275 °C - Pensky-Martens closed cup

Equipamento de proteção individual

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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