Merck
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32211

Sigma-Aldrich

Chloroform

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

Sinônimo(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
Número CAS:
Peso molecular:
119.38
Beilstein:
1731042
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.04

Nível de qualidade

200

Agency

USP/NF
reag. ISO
reag. Ph. Eur.

densidade de vapor

4.1 (vs air)

pressão de vapor

160 mmHg ( 20 °C)

grau

puriss. p.a.

teor

99.0-99.4% (GC)

forma

liquid

contém

~1% ethanol as stabilizer

technique(s)

RNA extraction: suitable

Impurezas

≤0.00001% free chlorine (Cl)
≤0.00005% free acid (as HCl)
≤0.0005% non-volatile matter
≤0.005% aldehydes and ketones (as CH3COCH3)
≤0.005% carbonyl compounds (as CO)
≤0.01% tetrachloroethene (GC)
≤0.01% tetrachloromethane (GC)
≤0.01% trichloroethene (GC)
≤0.01% water (Karl Fischer)
≤0.03% dichloromethane (GC)
0.6-1.0% ethanol (GC)

índice de refração

n20/D 1.445 (lit.)

pb

60.5-61.5 °C (lit.)

pf

−63 °C (lit.)

densidade

1.476-1.483 g/mL at 20 °C
1.492 g/mL at 25 °C (lit.)

traços de ânion

chloride (Cl-): ≤1 mg/kg

traços de cátion

Al: ≤0.5 mg/kg
B: ≤0.02 mg/kg
Ba: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.05 mg/kg
Sn: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES string

ClC(Cl)Cl

adequação

complies for appearance
complies for reaction against H2SO4
complies for suitability of determ. w. dithizone

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Descrição geral

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

Aplicação

Chloroform may be employed as a solvent for the following studies:
  • Extraction of RNA from plant cells.
  • Preparation of dioleoylphosphatidylcholine (DOPC) solution.
  • Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).
It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.
Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.

Outras notas

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table
The article number 32211-4X2.5L will be discontinued. Please order the single bottle 32211-2.5L which is physically identical with the same exact specifications.
The article number 32211-4X2.5L-M will be discontinued. Please order the single bottle 32211-2.5L-M which is physically identical with the same exact specifications.
The article number 32211-6X1L will be discontinued. Please order the single bottle 32211-1L which is physically identical with the same exact specifications.
The article number 32211-6X1L-M will be discontinued. Please order the single bottle 32211-1L-M which is physically identical with the same exact specifications.

Pictogramas

Skull and crossbonesHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Ponto de fulgor (ºF)

does not flash

Ponto de fulgor (ºC)

does not flash

Certificado de análise

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Certificado de origem

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Chloroform is not solvent but activator for cobalt complex catalyst of enantioselective borohydride reduction.
Kokura, Ai, et al.
Chemistry Letters (Jpn), 36(1), 26-27 (2006)
Gurman Singh Pall et al.
Nucleic acids research, 35(8), e60-e60 (2007-04-05)
The northern blot, or RNA gel blot, is a widely used method for the discovery, validation and expression analysis of small regulatory RNA such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA). Although it is straightforward and
Jameel A Feshitan et al.
Journal of colloid and interface science, 329(2), 316-324 (2008-10-28)
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields
Z V Leonenko et al.
Biochimica et biophysica acta, 1509(1-2), 131-147 (2000-12-19)
We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES)
G F Ames et al.
Journal of bacteriology, 160(3), 1181-1183 (1984-12-01)
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the

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