Merck
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496189

Sigma-Aldrich

Chloroform

biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sinônimo(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
Número CAS:
Peso molecular:
119.38
Beilstein:
1731042
Número MDL:
ID de substância PubChem:
NACRES:
NA.04

Nível de qualidade

100

grau

biotech. grade

densidade de vapor

4.1 (vs air)

pressão de vapor

160 mmHg ( 20 °C)

teor

≥99.8%

forma

liquid

contém

0.5-1.0% ethanol as stabilizer

Impurezas

<0.01% water

resíduo de evaporação

<0.0003%

cor

APHA: ≤10

índice de refração

n20/D 1.445 (lit.)

pH

5.2-6.4

pb

60.5-61.5 °C (lit.)

pf

−63 °C (lit.)

densidade

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

traços de cátion

Pb: ≤0.05 ppm

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Descrição geral

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Low water content, minimal residue and clean UV spectra are the special characteristics of this biotech grade solvent. It can be utilized for biotechnological processes like protein sequencing, peptide and oligonucleotide synthesis. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.

Aplicação

Chloroform may be used in the following processes:
  • Synthesis of colloidal hydrophobic CdSe core nanocrystals (quantum dots).
  • Preparing phospholipid solutions.
  • As a solvent in the photolysis reaction of ruthenium complexes.
  • Solvent for recrystallization.
  • Reference compound to detect the presence of chloroform in Tobacco smokes.
  • Extractant in solvent extraction process.

Embalagem

1, 2 L in Sure/Seal™

Pictogramas

Skull and crossbonesHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Ponto de fulgor (ºF)

does not flash

Ponto de fulgor (ºC)

does not flash

Certificado de análise

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Certificado de origem

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Systematic studies on the breakdown of p,p'-DDT in tobacco smokes. Presence of methyl chloride, dichloromethane, and chloroform in tobacco smokes.
Chopra NM and Sherman LR.
Analytical Chemistry, 44(6), 1036-1038 (1972)
Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots.
Sukhanova A, et al.
Protocol Exchange (2012)
Eagleson M.
Concise Encyclopedia Chemistry, 216-216 (1994)
Traces of phosgene in chloroform: Consequences for extraction of anthracyclines.
Maudens KE, et al.
Journal of Chromatography. B, Biomedical Applications, 848(2), 384-390 (2007)
The affinity of two antimicrobial peptides derived from bovine milk proteins for model lipid membranes.
Barzyk W, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 343(1), 104-110 (2009)

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