Merck
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C2432

Sigma-Aldrich

Chloroform

contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Sinônimo(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
Número CAS:
Peso molecular:
119.38
Beilstein:
1731042
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.04

Nível de qualidade

100

densidade de vapor

4.1 (vs air)

pressão de vapor

160 mmHg ( 20 °C)

teor

≥99.5%

forma

liquid

contém

100-200 ppm amylenes as stabilizer

technique(s)

RNA extraction: suitable

índice de refração

n20/D 1.445 (lit.)

pb

60.5-61.5 °C (lit.)

pf

−63 °C (lit.)

densidade

1.492 g/mL at 25 °C (lit.)

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Descrição geral

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. The product contains amylene as a stabilizer, since chloroform is susceptible to degradation with time. Amylene acts as a scavenger of free radicals formed during chloroform decomposition.

Aplicação

Chloroform has been used for the isolation of RNA from mammalian tissue.

Embalagem

25, 500 mL in glass bottle
1, 2.5 L in glass bottle

Pictogramas

Skull and crossbonesHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Ponto de fulgor (ºF)

does not flash

Ponto de fulgor (ºC)

does not flash

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What amount of amylenes are present in Product C2432, Chloroform, as a stabilizer?

    The concentration of amylenes is 100 - 300 ppm.

  7. Has Product C2432, Chloroform, been tested for DNase and RNase activity?

    This product has not been tested for DNase and RNase activity. However, it has been tested and found to be suitable for the extraction of nucleic acids based upon the evaluation of extracted DNA by agarose electrophoresis.

  8. How can Product C2432, Chloroform, be used to extract proteins from nucleic acids?

    A protocol for using phenol:chloroform or phenol:chloroform:isoamyl alcohol to extract proteins from nucleic acids is described in Molecular Cloning: A Laboratory Manual, vol. 3, pp E.3 - E.4 (1989).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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