03978

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Sinônimo(s): Methylene blue stain, Loeffler’s Methylene Blue, Ehrlich’s reagent III
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
• Número CAS: 61-73-4
• Peso molecular: 319.85
• Beilstein: 3641570
• Número MDL: MFCD00012111
• ID de substância PubChem: 329747853
• NACRES: NA.47

Propriedades

• Nível de qualidade: 100
• grau: for microscopy
• forma: liquid
• qualidade: concentrated, aqueous solution
• prazo de validade: ~3 yr
• concentração: 1.5% (1.5 g/ 100 mL)
• densidade: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Descrição

Descrição geral

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Aplicação

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter