Merck
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03978

Sigma-Aldrich

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Sinônimo(s):
Methylene blue stain, Loeffler’s Methylene Blue, Ehrlich’s reagent III
Empirical Formula (Hill Notation):
C16H18ClN3S
Número CAS:
Peso molecular:
319.85
Beilstein:
3641570
Número MDL:
ID de substância PubChem:
NACRES:
NA.47

Nível de qualidade

100

grau

for microscopy

forma

liquid

qualidade

concentrated, aqueous solution

prazo de validade

~3 yr

concentração

1.5% (1.5 g/ 100 mL)

densidade

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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Descrição geral

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.
Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Aplicação

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificado de análise

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Certificado de origem

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