Merck
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09978

Sigma-Aldrich

Ammonium sulfate

BioUltra, ≥99.0% (T)

Sinônimo(s):
Mascagnite
Fórmula linear:
(NH4)2SO4
Número CAS:
Peso molecular:
132.14
Número EC:
Número MDL:
eCl@ss:
38030408
ID de substância PubChem:
NACRES:
NA.26

linha de produto

BioUltra

teor

≥99.0% (T)

forma

crystals

Impurezas

insoluble matter, passes filter test

resíduo de ignição

≤0.01% (as SO4)

perda

≤0.1% loss on drying, 110 °C

pH

5.0-6.0 (25 °C, 1 M in H2O)

pf

>280 °C (dec.) (lit.)

solubilidade

H2O: 1 M at 20 °C, clear, colorless

densidade

1.77 g/mL at 25 °C (lit.)

traços de ânion

chloride (Cl-): ≤5 mg/kg
nitrate (NO3-): ≤50 mg/kg
phosphate (PO43-): ≤5 mg/kg

traços de cátion

Al: ≤5 mg/kg
As: ≤0.2 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤50 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤2 mg/kg
Sr: ≤5 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

Absorção UV

λ: 260 nm Amax: ≤0.037
λ: 280 nm Amax: ≤0.030

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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Aplicação

May be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins.

Outras notas

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose® chromatography of tRNAs using reverse salt gradients.

Informações legais

Sepharose is a registered trademark of Cytiva

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Mais documentos

Quotes and Ordering

T.D. Pollard
Methods in Enzymology, 85, 334-334 (1982)
Sepharose chromatography of transfer ribonucleic acids using reverse salt gradients.
G W Hatfield
Methods in enzymology, 59, 215-218 (1979-01-01)
Protein crystallization: the growth of large-scale single crystals.
G L Gilliland et al.
Methods in enzymology, 104, 370-381 (1984-01-01)
Simon Gareth Broadley et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 11), 1450-1459 (2012-10-24)
MshB, a zinc-based deacetylase, catalyses a step in the mycothiol biosynthetic pathway that involves the deacetylation of 1-O-(2-acetamido-2-deoxy-α-D-glucopyranosyl)-D-myo-inositol (GlcNAc-Ins), via cleavage of an amide bond, to 1-O-(2-amino-2-deoxy-α-D-glucopyranosyl)-D-myo-inositol (GlcN-Ins) and acetate. In this study, MshB was expressed, purified and crystallized. A
Kazunori Sugiura et al.
Biochemical and biophysical research communications, 457(3), 242-248 (2015-01-17)
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004))

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