Merck
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43815

Sigma-Aldrich

DL-Dithiothreitol

BioUltra, for molecular biology, ≥99.5% (RT)

Sinônimo(s):
threo-1,4-Dimercapto-2,3-butanediol, DTT, Cleland’s reagent
Fórmula linear:
HSCH2CH(OH)CH(OH)CH2SH
Número CAS:
Peso molecular:
154.25
Beilstein:
1719757
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

Nível de qualidade

200

grau

for molecular biology

linha de produto

BioUltra

teor

≥99.5% (RT)

forma

powder

Impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.3% 4,5-dihydroxy-1,2-dithiane

pH

4.0-6.5 (25 °C, 0.1 M in H2O)

pf

41-44 °C (lit.)

solubilidade

H2O: 0.1 M at 20 °C, clear, colorless

traços de ânion

sulfate (SO42-): ≤50 mg/kg

traços de cátion

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

Absorção UV

λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100

temperatura de armazenamento

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Categorias relacionadas

Aplicação

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Outras notas

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.

Pictogramas

CorrosionExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de análise

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Certificado de origem

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Fichas de informações

Mais documentos

Cotações e pedidos

Isolation of RNA using guanidinium salts.
R J MacDonald et al.
Methods in enzymology, 152, 219-227 (1987-01-01)
Pierre Larraufie et al.
Cell reports, 26(6), 1399-1408 (2019-02-07)
Bariatric surgery is widely used to treat obesity and improves type 2 diabetes beyond expectations from the degree of weight loss. Elevated post-prandial concentrations of glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and insulin are widely reported, but the importance
Christopher D Wiley et al.
Cell reports, 28(13), 3329-3337 (2019-09-26)
Cellular senescence irreversibly arrests cell proliferation, accompanied by a multi-component senescence-associated secretory phenotype (SASP) that participates in several age-related diseases. Using stable isotope labeling with amino acids (SILACs) and cultured cells, we identify 343 SASP proteins that senescent human fibroblasts
Gabriele A Fontana et al.
Nature communications, 10(1), 2535-2535 (2019-06-12)
Rif1 is involved in telomere homeostasis, DNA replication timing, and DNA double-strand break (DSB) repair pathway choice from yeast to human. The molecular mechanisms that enable Rif1 to fulfill its diverse roles remain to be determined. Here, we demonstrate that
Xueqian Liu et al.
The Journal of biological chemistry, 288(10), 7230-7240 (2013-01-25)
In Pichia pastoris, the peroxisomal targeting signal 2 (PTS2)-dependent peroxisomal matrix protein import pathway requires the receptor, Pex7, and its co-receptor Pex20. A conserved lysine (Lys(19)) near the N terminus of Pex20 is required for its polyubiquitination and proteasomal degradation

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