43815

DL-Dithiothreitol

BioUltra, for molecular biology, ≥99.5% (RT)

• Sinônimo(s): threo-1,4-Dimercapto-2,3-butanediol, DTT, Cleland’s reagent
• Fórmula linear: HSCH₂CH(OH)CH(OH)CH₂SH
• Número CAS: 3483-12-3
• Peso molecular: 154.25
• Beilstein: 1719757
• Número EC: 222-468-7
• Número MDL: MFCD00004877
• ID de substância PubChem: 57650195
• NACRES: NA.25

Propriedades

• Nível de qualidade: 200
• grau: for molecular biology
• linha de produto: BioUltra
• teor: ≥99.5% (RT)
• forma: powder
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.3% 4,5-dihydroxy-1,2-dithiane
• pH: 4.0-6.5 (25 °C, 0.1 M in H₂O)
• pf: 41-44 °C (lit.)
• solubilidade: H₂O: 0.1 M at 20 °C, clear, colorless
• traços de ânion: sulfate (SO₄^2-): ≤50 mg/kg
• traços de cátion: Al: ≤5 mg/kg; As: ≤0.5 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.400; λ: 280 nm A_max: 0.100
• temperatura de armazenamento: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Descrição

Aplicação

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Outras notas

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.

Informações de segurança

• Pictogramas: GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H302 - H315 - H318
• Declarações de precaução: P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves