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Merck

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Pular para

47608

Formaldeído

Molecular Biology, BioReagent, ≥36.0% in H2O (T)

Sinônimo(s):

Formalina

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Sobre este item

Fórmula linear:
HCHO
Número CAS:
Peso molecular:
30.03
UNSPSC Code:
12352114
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1209228
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable
Vapor pressure:
52 mmHg ( 37 °C)
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grade

Molecular Biology

Quality Segment

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)

product line

BioReagent

form

liquid

autoignition temp.

572 °F

contains

~10% methanol as stabilizer

concentration

≥36.0% in H2O (T), 36.0-38.0% (aldehyde method)

technique(s)

electrophoresis: suitable

impurities

≤0.03% free acid (as HCOOH)

ign. residue

≤0.05% (as SO4)

pH

2.8-4.0

density

1.09 g/mL at 20 °C, 1.09 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg, sulfate (SO42-): ≤20 mg/kg

cation traces

Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Na: ≤100 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Zn: ≤1 mg/mL

λ

2 M in H2O

UV absorption

λ: 260 nm Amax: 0.01, λ: 280 nm Amax: 0.01

suitability

in accordance for electrophoresis of RNA in agarose gels

storage temp.

room temp

SMILES string

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Application

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
  • in fixing zebra fish larvae for neutrophil staining.

Biochem/physiol Actions

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Disclaimer

may contain precipitate (paraformaldehyde)

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Este Item
F877525254915512
Formaldeído Molecular Biology, BioReagent, ≥36.0% in H2O (T)

Sigma-Aldrich

47608

Formaldeído

Formaldeído Molecular Biology, 36.5-38% in H2O

Sigma-Aldrich

F8775

Formaldeído

Formaldeído ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

Sigma-Aldrich

252549

Formaldeído

Formaldeído meets analytical specification of USP, ≥34.5 wt. %

Sigma-Aldrich

15512

Formaldeído

description

Molecular Biology, BioReagent, ≥36.0% in H2O (T)

description

Molecular Biology, 36.5-38% in H2O

description

ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

description

meets analytical specification of USP, ≥34.5 wt. %

technique(s)

electrophoresis: suitable

technique(s)

ChIP: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

-

technique(s)

-

grade

Molecular Biology

grade

Molecular Biology

grade

ACS reagent

grade

-

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

-

UV absorption

λ: 260 nm Amax: 0.01, λ: 280 nm Amax: 0.01

UV absorption

-

UV absorption

-

UV absorption

-

vapor pressure

52 mmHg ( 37 °C)

vapor pressure

52 mmHg ( 37 °C)

vapor pressure

52 mmHg ( 37 °C)

vapor pressure

52 mmHg ( 37 °C)


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signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Classe de armazenamento

3 - Flammable liquids

wgk

WGK 3

flash_point_f

133.0 °F - closed cup

flash_point_c

56.11 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Questions

  1. How long can this product be stored and what should be the storage conditions (ambient, refrigerated, frozen)? Does opening of the bottle impacts the expiry date?

    1 answer
    1. This product should be stored at ambient temperature in a tightly closed container, in a dry, well ventilated area away from heat and ignition sources. Provided that the material is stored properly and not contaminated, opening the bottle does impact the assigned expiry date.

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