49767

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

• Synonym(s): 1,2,3-Propanetriol, Glycerin
• Linear Formula: HOCH₂CH(OH)CH₂OH
• CAS Number: 56-81-5
• Molecular Weight: 92.09
• Beilstein: 635685
• Número EC: 200-289-5
• Número MDL: MFCD00004722
• ID de substância PubChem: 57651220
• NACRES: NA.25

Properties

• grau: for molecular biology
• Nível de qualidade: 200
• densidade de vapor: 3.1 (vs air)
• pressão de vapor: <1 mmHg ( 20 °C)
• linha de produto: BioUltra
• teor: ≥99.5% (GC)
• forma: viscous liquid
• temperatura de autoignição: 698 °F
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• resíduo de ignição: ≤0.1%
• índice de refração: n20/D 1.474 (lit.); n20/D 1.474
• pH: 5.5-8 (25 °C, 5 M in H₂O)
• pb: 182 °C/20 mmHg (lit.)
• pf: 20 °C (lit.)
• solubilidade: H₂O: 5 M at 20 °C, clear, colorless
• densidade: 1.25 g/mL (lit.)
• traços de ânion: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• traços de cátion: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤5 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• SMILES string: OCC(O)CO
• λ: 5 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.07
• InChI: 1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
• InChI key: PEDCQBHIVMGVHV-UHFFFAOYSA-N

Description

Descrição geral

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Aplicação

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Ações bioquímicas/fisiológicas

Glycerol has been used as

• a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• a fuel during designing enzymatic biofuel cell.
• a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
• a matrix for fast atom bombardment MS.
• may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Outras notas

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Safety Information

• Código de classe de armazenamento: 10 - Combustible liquids
• WGK: WGK 1
• Ponto de fulgor (ºF): 390.2 °F - Pensky-Martens closed cup
• Ponto de fulgor (ºC): 199 °C - Pensky-Martens closed cup
• Equipamento de proteção individual: Eyeshields, Gloves