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49767

Sigma-Aldrich

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

Sinônimo(s):
1,2,3-Propanetriol, Glycerin
Fórmula linear:
HOCH2CH(OH)CH2OH
Número CAS:
Peso molecular:
92.09
Beilstein:
635685
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

Nível de qualidade

200

grau

for molecular biology

densidade de vapor

3.1 (vs air)

pressão de vapor

<1 mmHg ( 20 °C)

linha de produto

BioUltra

teor

≥99.5% (GC)

forma

viscous liquid

temperatura de autoignição

698 °F

Impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

resíduo de ignição

≤0.1%

índice de refração

n20/D 1.474 (lit.)
n20/D 1.474

pH

5.5-8 (25 °C, 5 M in H2O)

pb

182 °C/20 mmHg (lit.)

pf

20 °C (lit.)

solubilidade

H2O: 5 M at 20 °C, clear, colorless

densidade

1.25 g/mL (lit.)

traços de ânion

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

traços de cátion

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤5 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES string

OCC(O)CO

λ

5 M in H2O

Absorção UV

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.04

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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Descrição geral

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Aplicação

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Embalagem

1 L in glass bottle
100, 250 mL in glass bottle

Ações bioquímicas/fisiológicas

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.
Glycerol has been used as
  • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
  • a fuel during designing enzymatic biofuel cell.
  • a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • a matrix for fast atom bombardment MS.
  • may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Outras notas

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Storage Class Code

10 - Combustible liquids

WGK Alemanha

WGK 1

Ponto de fulgor (ºF)

390.2 °F - Pensky-Martens closed cup

Ponto de fulgor (ºC)

199 °C - Pensky-Martens closed cup

Equipamento de proteção individual

Eyeshields, Gloves

Certificado de análise

Certificado de origem

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the viscosity of glycerol?

    The viscosity of glycerol is 648 Centistokes at 20 Deg C (68 Deg F).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

R.C. Ogden et al.
Methods in Enzymology, 152, 79-79 (1987)
Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage.
H Miller
Methods in enzymology, 152, 145-170 (1987-01-01)
Shelley L Forrest et al.
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T W Chew et al.
Oncogene, 33(21), 2717-2727 (2013-07-03)
RAS and Rho small GTPases are key molecular switches that control cell dynamics, cell growth and tissue development through their distinct signaling pathways. Although much has been learnt about their individual functions in both cell and animal models, the physiological...

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