Merck
All Photos(1)

5006

Sigma-Aldrich

PureCol

protein from bovine hide, suitable for cell culture

Número EC:
NACRES:
NA.75

Nível de qualidade

100

fonte biológica

protein from bovine hide

esterilidade

sterile-filtered

teor

99.9%

forma

lyophilized

embalagem

pkg of 15 mg

technique(s)

cell culture | mammalian: suitable

enviado em

wet ice

temperatura de armazenamento

−20°C

Related Categories

Descrição geral

PureCol collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol, lyophilized form is not recommended for the formation of a solid gel. PureCol collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Aplicação

PureCol has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Nota de preparo

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Informações legais

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service