Merck
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56748

Sigma-Aldrich

Imidazole

BioUltra, for molecular biology, ≥99.5% (GC)

Synonym(s):
1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
Empirical Formula (Hill Notation):
C3H4N2
CAS Number:
Molecular Weight:
68.08
Beilstein:
103853
Número EC:
Número MDL:
eCl@ss:
39161001
ID de substância PubChem:
NACRES:
NA.25

grau

for molecular biology

Nível de qualidade

200

pressão de vapor

<1 mmHg ( 20 °C)

linha de produto

BioUltra

teor

≥99.5% (GC)

forma

flakes
powder or crystals

Impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.2% water

resíduo de ignição (900 °C)

≤0.05%

pH

9.5-11.0 (25 °C, 0.1 M in H2O)

pKa (25 °C)

6.95

pb

256 °C (lit.)

pf

88-91 °C (lit.)

solubilidade

H2O: 0.1 M at 20 °C, clear, colorless

traços de ânion

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

traços de cátion

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

c1c[nH]cn1

λ

0.1 M in H2O

Absorção UV

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.10

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

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Aplicação

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.
Excellent for buffers in the range of pH 6.2-7.8

Outras notas

Review: As RNase model

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Código de classe de armazenamento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Ponto de fulgor (ºF)

293.0 °F - closed cup

Ponto de fulgor (ºC)

145 °C - closed cup

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificate of Analysis

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Certificate of Origin

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