646547

Tris(2-carboxyethyl)phosphine hydrochloride solution

0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

• Sinônimo(s): TCEP
• Empirical Formula (Hill Notation): C₉H₁₅O₆P · HCl
• Número CAS: 51805-45-9
• Peso molecular: 286.65
• Número MDL: MFCD00145469
• ID de substância PubChem: 24883469
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• concentração: 0.5 M
• índice de refração: n20/D 1.367
• pH: 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
• densidade: 1.041 g/mL at 25 °C
• SMILES string: Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

Descrição

Descrição geral

Tris (2-carboxyethyl) phosphine (TCEP) is very effective in cleaving disulfide bonds in aqueous solution. It dissolves in water and is odorless, unlike other trialkylphosphines (tributylphosphine). It is also less toxic than 2-mercaptoethanol. These advantages make it better than the other reducing agents.

It belongs to the trialkylphosphine class.

Aplicação

Tris (2-carboxyethyl) phosphine (TCEP) can be used in several downstream applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. It prevents oxidation of protein samples, which makes it a useful buffer component as it helps to preserve enzymatic activity. It has been used in the reduction and measurement of glutathione.

Tris (2-carboxyethyl) phosphine (TCEP) has also been used:

• to cleave cysteine residues in a synthetic peptide
• in reduction buffer for RNA Sequential Probing of Targets (SPOTs) imaging
• for the reduction of oligonucleotides
• as reducing agent during mitochondrial isolation

Embalagem

10×1 mL in ampule

Ações bioquímicas/fisiológicas

Tris(2-carboxyethyl)phosphine hydrochloride solution reduces the disulfide bonds and leaves other functional groups intact in proteins.

As a non-mercaptan reducing agent, it avoids the toxicity inherent in thiol-containing compounds. It is capable of disrupting the botulinum neurotoxin B heavy-chain/light-chain complex that is held together by a single disulfide bond, and that is responsible for endocytosis, and ultimately the toxicity, of the toxin. Since disulfide-coupled subunits are characteristic of many toxins (e.g., ricin, snake venom, and all BoNT serotypes), it may be useful as a rescue prophylactic in cases of toxin administration.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315 - H319 - H335
• Declarações de precaução: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 10 - Combustible liquids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter