646563

DL-Dithiothreitol solution

1 M in H₂O

• Synonym(s): (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
• Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• Número MDL: MFCD00004877
• ID de substância PubChem: 24883472
• NACRES: NA.25

Properties

• Nível de qualidade: 100
• reaction suitability: reagent type: reductant
• concentração: 1 M in H₂O
• densidade: 1.04 g/mL at 20 °C; 1.035 g/mL at 25 °C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

Descrição geral

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.

Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Aplicação

DL-Dithiothreitol solution has been used:

• in western blot analysis
• as a supplement in lysis buffer to study its effects on sperm lysis
• as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
• in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Características e benefícios

Low toxicity and reduced odor compared to other thiol compounds.

Safety Information

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H315 - H318
• Declarações de precaução: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Classificações de perigo: Eye Dam. 1 - Skin Irrit. 2
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• WGK: WGK 2
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter