Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Origem de linhagem celular
Descrição de linhagem celular
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Bonn, Germany
Tissue collection date: Aug. 26, 2009
Passage number reprogrammed: P4
Vector type: Integrating
Virus type: Retrovirus
Have the reprogramming vectors been silenced: Unknown
Analysis of Undifferentiated Cells
Marker expression: TRA 1-81(+)SSEA-3(+)TRA 1-60(+)POU5F1 (OCT-4)(+)
Ectoderm: Neuroepithelial Stem Cell
In vivo teratoma
In vitro directed differentiation
Endoderm: In vivo teratoma
Mesoderm: In vivo teratoma
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: -
Hepatitis B: Negative
Hepatitis C: Negative
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Note: EAUA and CLIP must be completed before order fulfillment
Rotina de subcultura
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.