Merck
All Photos(2)

71768

Sigma-Aldrich

4-Nitrofenil fosfato

suitable for enzyme immunoassay, ≥99.0% (enzymatic)

Synonym(s):
4-Nitrofenil fosfato dissódico, p-Nitrofenil fosfato, pNPP
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
Beilstein:
5324661
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.83

Nível de qualidade

Ensaio

≥99.0% (enzymatic)

forma

powder or crystals

technique(s)

enzyme immunoassay: suitable

Impurezas

≤0.1% free nitrophenol

temperatura de armazenamento

2-8°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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This Item
SRE0026N9389N2765
assay

≥99.0% (enzymatic)

assay

≥97% (HPLC)

assay

-

assay

-

form

powder or crystals

form

powder

form

tablet

form

tablet

technique(s)

enzyme immunoassay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

400

Quality Level

300

Quality Level

300

impurities

≤0.1% free nitrophenol

impurities

-

impurities

-

impurities

-

Descrição geral

Substrato cromogênico para determinação de fosfatases alcalinas e ácidas. A reação gera um produto final solúvel de cor amarela. A reação pode ser interrompida com NaOH 3 N e lida em 405 nm.

Aplicação

O sal 4-nitrofenil fosfato dissódico hexa-hidratado foi usado:
  • como substrato para medir os parâmetros cinéticos da atividade de fosfatase dos homólogos de tensina e fosfatase deletados no cromossomo 10 (PTEN)
  • como substrato para ensaio da atividade da fosfatase ácida
  • como substrato para ensaio de anticorpos ligados com conjugado anti-fosfatase alcalina de coelho/humana

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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