83264

HEPES buffer solution

1 M in H₂O

• Sinônimo(s): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), HEPES solution
• Número CAS: 7365-45-9
• Número MDL: MFCD00006158
• eCl@ss: 32129211
• ID de substância PubChem: 57652951
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• forma: liquid
• concentração: 1 M in H₂O; 238 g/L HEPES in H₂O
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5±0.5
• useful pH range: 6.8-8.2
• densidade: 1.07 g/mL at 20 °C
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrição

Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicação

HEPES has been used:

• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
• As a component of dissection buffer for culturing rat cortical neurons
• Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
• As a component of Danieu′s aquarium water used in agarose gel preparation

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)