Merck
Todas as fotos(3)

87748

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥98.0% (NT)

Sinônimo(s):
TMB substrate, TMB, Sure Blue TMB, BM blue, TMB Blotting Plus
Fórmula linear:
[-C6H2(CH3)2-4-NH2]2
Número CAS:
Peso molecular:
240.34
Beilstein:
2808541
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.83

Nível de qualidade

300

teor

≥98.0% (NT)

forma

powder

technique(s)

UV/Vis spectroscopy: suitable

pf

166-171 °C
168-171 °C (lit.)

temperatura de armazenamento

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

Inchi Key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Aplicação

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
  • enzyme-immunoassay (EIA)
  • indirect competitive enzyme-linked immunosorbent assay (ELISA)
  • sandwich enzyme-linked immunosorbent assay

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de análise

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Certificado de origem

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