93773

TWEEN^® 20

viscosity 250-450 mPa.s (25 °C)

• Synonym(s): Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
• CAS Number: 9005-64-5
• Número MDL: MFCD00165986
• NACRES: NA.21

Properties

• descrição: non-ionic
• Nível de qualidade: 200
• forma: viscous liquid
• peso molecular: ~1228
• technique(s): DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• índice de refração: n20/D 1.468 (lit.); n20/D 1.471
• viscosidade: 250-450 mPa.s(25 °C)
• Valor HLB: 16.7±1.0
• densidade: 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.)
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• InChI key: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Description

Descrição geral

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]

Aplicação

TWEEN 20 is widely used in several biochemical applications. It has been used:

• As an emulsifying agent for the preparation of stable oil-in-water emulsions[2]
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)[3]
• As a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%[4][5][6]
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• For rehydrating tissue before in situ hybridization[7]

Non-ionic detergent

Atenção

may get turbid on storage, heat to clear

Informações legais

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Código de classe de armazenamento: 10 - Combustible liquids
• WGK: WGK 1
• Ponto de fulgor (ºF): 527.0 °F - Pensky-Martens closed cup
• Ponto de fulgor (ºC): 275 °C - Pensky-Martens closed cup
• Equipamento de proteção individual: Eyeshields, Gloves