A1720

Sigma-Aldrich

G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Sinônimo(s):
Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
Número CAS:
Peso molecular:
692.71
Número MDL:
ID de substância PubChem:
NACRES:
NA.76

Nível de qualidade

200

linha de produto

BioReagent

forma

powder

atividade óptica

[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))

potência

≥720 μg per mg (Dried basis)

peso molecular

692.7

embalagem

pkg of 1 g
pkg of 25 g
pkg of 5 g

aplicação(ões)

cell culture | mammalian: suitable

cor

white to off-white

solubilidade

H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)

Modo de ação

protein synthesis | interferes

espectro de atividade do antibiótico

Gram-negative bacteria

temperatura de armazenamento

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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Descrição geral

Chemical structure: aminoglycoside
G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.

Aplicação

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Embalagem

1, 5, 25 g in glass bottle

Ações bioquímicas/fisiológicas

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.

Atenção

The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Nota de preparo

G418 powder is soluble in water at 100 mg/mL.

Outras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

hazcat

Resp. Sens. 1 - Skin Sens. 1

storage_class_code

13 - Non Combustible Solids

WGK Alemanha

WGK 3

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Certificado de origem

G H Wagman et al.
Antimicrobial agents and chemotherapy, 6(2), 144-149 (1974-08-01)
Antibiotic G-418 is a new aminoglycoside produced as the major component by a new species of Micromonospora, M. rhodorangea NRRL 5326. The antibiotic is prepared by submerged fermentation in a soybean-dextrin medium. Antibiotic G-418 is adsorbed on a cationic-exchange resin...
D C Eustice et al.
Antimicrobial agents and chemotherapy, 26(1), 53-60 (1984-07-01)
Tetrahymena thermophila is a eucaryotic organism that is highly susceptible to growth inhibition by aminoglycoside antibiotics. Concentrations of paromomycin, gentamicin G418, and hygromycin B at 22, 10, and 17 microM, respectively, inhibited growth by 50%. A combination of in vitro...
Serene R Lai et al.
Methods in molecular biology (Clifton, N.J.), 405, 31-37 (2008-03-29)
RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today's cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a...
Hillary C Valley et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 18(4), 476-483 (2018-12-20)
Assessment of approved drugs and developmental drug candidates for rare cystic fibrosis (CF)-causing variants of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) requires abundant material from relevant models. Isogenic cell lines harboring CFTR variants in the native genomic context were...
Nikolay P Kandul et al.
Nature communications, 11(1), 2106-2106 (2020-05-02)
Here, we describe a drug-inducible genetic system for insect sex-separation that demonstrates proof-of-principle for positive sex selection in D. melanogaster. The system exploits the toxicity of commonly used broad-spectrum antibiotics geneticin and puromycin to kill the non-rescued sex. Sex-specific rescue...
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