Todas as fotos(6)



Bovine Serum Albumin

heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

Albumin bovine serum, Bovine albumin, BSA
Número CAS:
Número EC:
Número MDL:

Nível de qualidade


fonte biológica





lyophilized powder

peso molecular

~66 kDa

purificado por

heat shock fractionation


poly bottle of


USA origin


ELISA: suitable
western blot: suitable


essentially globulin free
protease, none detected






water: soluble (40 mg/ml)

nº de adesão UniProt

temperatura de armazenamento


Gene Information

bovine ... ALB(280717)

Procurando produtos similares? Visit Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
  • Protein quantitation (as a standard)
  • Immunochemistry (as a blocking agent)
  • Cell culture (as a nutrient)

This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques that include:
a) Enzyme immunoassay (EIA)
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)


Bovine Serum Albumin has been used in luminescence assays.


10, 50, 100, 500 g in poly bottle

Ações bioquímicas/fisiológicas

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Características e benefícios

  • Heat shock fractionated
  • Protease-free
  • Essentially globulin-free

Nota de preparo

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Código de classe de armazenamento

11 - Combustible Solids



Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

  1. Is Product A3059, Albumin from bovine serum (BSA), suitable for use in ELISA?

    We have not tested A3059 for specific use in ELISA. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in ELISA, by reducing a potential source of background.

  2. Is Product A3059, Albumin from bovine serum (BSA), suitable for use as a blocking agent in immunoblotting?

    We have not tested A3059 for specific use in immunoblotting as a blocking agent, in quality control. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in blotting experiments, by reducing a potential source of background.  The Technical Bulletin states that "Globulin free albumins are suitable for use in applications where no other proteins should be present".

  3. What is the fatty acid content of Product A3059, Albumin from bovine serum (BSA)?  Can it be considered fatty acid free?

    The fatty acid content of A3059 is not tested in quality control, and it is not reported on our Certificate of Analysis. We would not refer to this product as "fatty acid free".

  4. Is Product A3059, Albumin from bovine serum (BSA), endotoxin-free?

    We have not tested A3059 for endotoxin content. Endotoxin-tested BSA products would include A4919, A9306, A9430, A2934, A9306, A9543, or A3675.

  5. Is Product A3059, Albumin from bovine serum (BSA), acetylated to inactivate nucleases? 

    A3059 is not acetylated. We do not test for the presence of nucleases. Acetylated BSA products would be B2518 or B8894.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Dual luminescence-based reporter gene assay for luciferase and beta-galactosidase
Martin C S, et al.
Biotechniques, 520-524 (1996)
Priyank A Shenoy et al.
Frontiers in pharmacology, 9, 495-495 (2018-06-06)
In the majority of patients with breast cancer in the advanced stages, skeletal metastases are common, which may cause excruciating pain. Currently available drug treatments for relief of breast cancer-induced bone pain (BCIBP) include non-steroidal anti-inflammatory drugs and strong opioid
Edith L Pfister et al.
Human gene therapy, 29(6), 663-673 (2017-12-07)
Huntington's disease (HD) is a fatal neurodegenerative disease caused by a genetic expansion of the CAG repeat region in the huntingtin (HTT) gene. Studies in HD mouse models have shown that artificial miRNAs can reduce mutant HTT, but evidence for
Amanda Phuong Tran et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(23), 5399-5414 (2018-05-16)
Severed axon tips reform growth cones following spinal cord injury that fail to regenerate, in part, because they become embedded within an inhibitory extracellular matrix. Chondroitin sulfate proteoglycans (CSPGs) are the major axon inhibitory matrix component that is increased within
Ming Shen et al.
Autophagy, 13(8), 1364-1385 (2017-06-10)
Oxidative stress-induced granulosa cell (GCs) death represents a common reason for follicular atresia. Follicle-stimulating hormone (FSH) has been shown to prevent GCs from oxidative injury, although the underlying mechanism remains to be elucidated. Here we first report that the suppression

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica