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A3403

Sigma-Aldrich

α-Amylase from Bacillus licheniformis

greener alternative

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

Sinônimo(s):
1,4-α-D-Glucan-glucanohydrolase, Termamyl® 120
Número CAS:
Número da licença da enzima:
Número MDL:
eCl@ss:
32160410
NACRES:
NA.54

Nível de qualidade

200

tipo

Type XII-A

forma

saline solution

specific activity

≥500 units/mg protein (biuret)

peso molecular

57.6 kDa

características do produto alternativo mais ecológico

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

categoria alternativa mais ecológica

Enabling

temperatura de armazenamento

2-8°C

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Descrição geral

α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)8 structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Aplicação

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Ações bioquímicas/fisiológicas

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Atenção

Reported to be heat stable at temperatures as high as ∼90 °C.

Definição da unidade

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

forma física

Aqueous solution containing sodium chloride and sucrose

Informações legais

A product of Novozyme Corp.
Termamyl is a registered trademark of Novozymes Corp.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK Alemanha

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificado de análise

Certificado de origem

Zsofia Botyanszki et al.
Biotechnology and bioengineering, 112(10), 2016-2024 (2015-05-08)
Biocatalytic transformations generally rely on purified enzymes or whole cells to perform complex transformations that are used on industrial scale for chemical, drug, and biofuel synthesis, pesticide decontamination, and water purification. However, both of these systems have inherent disadvantages related...
Indira A Rasiah et al.
Applied and environmental microbiology, 75(7), 2012-2016 (2009-02-10)
Industrial enzymes are often immobilized via chemical cross-linking onto solid supports to enhance stability and facilitate repeated use in bioreactors. For starch-degrading enzymes, immobilization usually places constraints on enzymatic conversion due to the limited diffusion of the macromolecular substrate through...
Fan Zhang et al.
Molecules (Basel, Switzerland), 24(7) (2019-04-13)
Isomaltose-oligosaccharides (IMOs), as food ingredients with prebiotic functionality, can be prepared via enzymatic synthesis using α-glucosidase. In the present study, the α-glucosidase (GSJ) from Geobacillus sp. strain HTA-462 was cloned and expressed in Escherichia coli BL21 (DE3). Recombinant GSJ was...
Xinyi Huang et al.
Tree physiology, 40(5), 652-666 (2020-02-23)
Globally, soil salinization is becoming increasingly prevalent, due to local hydrogeologic phenomena, climate change and anthropogenic activities. This has significantly curtailed current world food production and limits future production potential. In the prairie region of North America, sulfate salts, rather...
Michaelle Chokki et al.
Foods (Basel, Switzerland), 9(4) (2020-04-09)
Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and...

Protocolos

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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