Merck
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A3678

Sigma-Aldrich

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

Sinônimo(s):
Ammonium peroxydisulfate, PER, Ammonium peroxodisulfate, APS, AP
Fórmula linear:
(NH4)2S2O8
Número CAS:
Peso molecular:
228.20
Número EC:
Número MDL:
eCl@ss:
38030421
ID de substância PubChem:
NACRES:
NB.22

Nível de qualidade

200

grau

for molecular biology

densidade de vapor

7.9 (vs air)

teor

≥98%

forma

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

traços de ânion

chloride (Cl-): <10 ppm

traços de cátion

Fe: <10 ppm
heavy metals (as Pb): <50 ppm

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

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Descrição geral

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Aplicação

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.
Catalyst for acrylamide gel polymerization.

Embalagem

25, 100 g in poly bottle

Nota de análise

Tested for use in acrylamide polymerization.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Product Information Sheet

Mais documentos

Quotes and Ordering

Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.
Chiang MY et al.
Biomaterials, 34, 9754-9754 (2013)
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
Fancy DA and Kodadek T
Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)
Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.
Liao IC et al.
Advances in Functional Materials, 23, 5833-5833 (2013)
Maurer HR
Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (1978)
Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.
Alvarez-Gonzalez CA, et al.
Fish Physiology and Biochemistry, 36, 29-29 (2010)

Artigos

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field.

Bis Tris Polyacrylamide Gel Electrophoresis Technology

Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.

Protocolos

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

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