A9169

Anti-IgG de coelho (molécula inteira)–peroxidase

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, Anti Rabbit Antibody Sigma
• Número MDL: MFCD00162788
• NACRES: NA.46

Propriedades

• fonte biológica: goat
• conjugado: peroxidase conjugate
• forma do anticorpo: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• species reactivity: rabbit
• technique(s): direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400; western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)
• enviado em: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

Especificidade

Specificity of the Peroxidase Conjugated Anti-Rabbit IgG antibodies is determined by immunoelectrophoresis (IEP) versus normal rabbit serum and rabbit IgG.

Imunogênio

purified rabbit IgG

Aplicação

A coimunoprecipitação e a análise por western blot dos lisados de células C33A foram realizados usando IgG anti-coelho de cabra conjugada com HRP como anticorpo secundário.

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.

Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.

Ações bioquímicas/fisiológicas

Immunoglobulins are glycoprotein antibodies that modulate several immune responses. Immunoglobulin G participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota de preparo

Prepared by two-step glutaraldehyde method.

Armazenamento e estabilidade

For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable