A9528

Amphotericin B solubilized

powder, γ-irradiated, BioXtra, suitable for cell culture

• Synonym(s): Amphotericin B from Streptomyces sp., Fungizone
• Empirical Formula (Hill Notation): C₄₇H₇₃NO₁₇
• CAS Number: 1397-89-3
• Molecular Weight: 924.08
• Beilstein: 78342
• Número MDL: MFCD00877763
• eCl@ss: 34010400
• ID de substância PubChem: 24891468

Properties

• Nível de qualidade: 300
• esterilidade: γ-irradiated
• linha de produto: BioXtra
• forma: powder
• technique(s): cell culture | mammalian: suitable
• espectro de atividade do antibiótico: fungi; yeast
• Modo de ação: cell membrane | interferes
• temperatura de armazenamento: 2-8°C
• SMILES string: C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
• InChI: 1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• InChI key: APKFDSVGJQXUKY-INPOYWNPSA-N

Description

Descrição geral

Chemical structure: polyene

Aplicação

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Ações bioquímicas/fisiológicas

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Embalagem

Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone.

Componentes

This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.

Atenção

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Nota de preparo

Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.

It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).

Outras notas

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.

The actual solid weight of the packaged product is calculated according to the UV purity.

Kit Components Only

• Amphotericin B ~ 45 %
• Sodium deoxycholate ~ 35 %
• Sodium phosphate balance

Safety Information

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H335
• Classificações de perigo: STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 13 - Non Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves