Merck
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C0887

Sigma-Aldrich

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, 1,000-2,000 units/mg protein (E1%/280)

Synonym(s):
Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
CAS Number:
Enzyme Commission number:
Número MDL:
NACRES:
NA.54

fonte biológica

fungus (Caldariomyces fumago)

Nível de qualidade

200

forma

buffered aqueous suspension

specific activity

1,000-2,000 units/mg protein (E1%/280)

peso molecular

42 kDa

razão de absorbância

RZ ~1.0

temperatura de armazenamento

2-8°C

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Embalagem

1250 units in poly bottle

Aplicação

A useful alternative to lactoperoxidase for 131I ion labeling studies, for bromination of proteins, and for 36Cl labeling of macromolecules in long-term isolation procedures.

Ações bioquímicas/fisiológicas

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H2O2 decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).

Definição da unidade

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H2O2.

forma física

Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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