Merck
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C2010

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV, lyophilized powder

Synonym(s):
Con A
CAS Number:
Número EC:
Número MDL:
NACRES:
NA.32

tipo

Type IV

Nível de qualidade

300

forma

lyophilized powder

potência

<64 μg per mL agglutination activity

Impurezas

Carbohydrate, essentially free
salt, essentially free

temperatura de armazenamento

−20°C

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Descrição geral

Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.

Aplicação

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
  • for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
  • for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
  • for the stimulation of spleen in mitogen assays

Embalagem

25 mg in poly bottle
100, 250 mg in glass bottle
1 g in glass bottle

Ações bioquímicas/fisiológicas

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Qualidade

Highly purified by specific affinity chromatography

Nota de análise

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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