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C2124

Sigma-Aldrich

Collagen solution from bovine skin

6 mg/mL, sterile-filtered, BioReagent, suitable for cell culture, and for 3D matrix formation.

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Número MDL:

fonte biológica

bovine skin

Nível de qualidade

esterilidade

sterile-filtered

linha de produto

BioReagent

forma

liquid

embalagem

pkg of 50 mL

concentração

6 mg/mL

technique(s)

cell culture | mammalian: suitable

cobertura de superfície

6‑10 μg/cm2

atividade externa

endotoxin ≤1.0 μmole/min-mg protein

enviado em

wet ice

temperatura de armazenamento

2-8°C

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Este Item
C4243C8919C6745
Sigma-Aldrich

Sigma-Aldrich

C2124

Collagen solution from bovine skin

Sigma-Aldrich

Sigma-Aldrich

C4243

Collagen solution from bovine skin

Sigma-Aldrich

Sigma-Aldrich

C8919

Collagen from calf skin

sterility

sterile-filtered

sterility

sterile-filtered

sterility

aseptically processed

sterility

sterile-filtered

form

liquid

form

liquid

form

solution (0.1% solution in 0.1 M acetic acid)

form

solution

packaging

pkg of 50 mL

packaging

pkg of 100 mL, pkg of 20 mL

packaging

pkg of 20 mL

packaging

pkg of 1 mL

concentration

6 mg/mL

concentration

2.9-3.2 mg/mL

concentration

(0.1% solution in 0.1 M acetic acid)

concentration

0.3 mg/mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Descrição geral

In mammals, collagen type 1 is a widely expressed fibrous protein. It constitutes to one-third of the total protein in humans. Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix, and is also found in arterial walls, tendons, ligaments, skin, cornea, bone and dentin. Collagen structure possesses a triple helix polypeptide strand.

Aplicação

Collagen solution from bovine skin has been used as a substrate in cell adhesion assay.
This highly purified solution is suitable for 3-D matrix formation in cell culture. 3-D collagen gels imitate the in vivo cell physiology better than traditional 2D systems and has been proven successful for several cell types including cardian and corneal fibroblasts, depatic stellate cells, and neuroblastoma cells. Such 3-D gels are also useful in studies of mechanotransduction, cell signaling involving the transformation of mechanical signals into biochemical signals

Ações bioquímicas/fisiológicas

Collagen type 1 is a versatile building material required for tissue elasticity and maintains the stability and strength in a variety of tissues. Collagen is also present in byssus thread of invertebrates, which helps in surface attachment. Defective collagen affects the biomechanical property of arterial tissue.
In 3D environments, cell extensions can use integrins on cell surfaces to activate specific signaling pathways and integran-independent mechanical interactions resulting from the entanglement of matrix fibrils is possible.

Componentes

Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. As a heterodimer composed of two a1 chains and one a2 chains, it spontaneously forms a triple helix scaffold at neutral pH and 37°C.

Nota de preparo

This product is prepared from type I bovine collagen purified and extracted from skin and contains a high monomer content. The raw collagen used to prepare this product has been isolated from a closed herd and purified with a GMP manufacturing process that includes inactivation of any possible prion or viral contamination. As supplied, it is a 6 mg/mL aqueous solution in 0.01 M HCl at a pH of 2.0.

Outras notas

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Código de classe de armazenamento

10 - Combustible liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable


Certificates of Analysis (COA)

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1000309185

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Artigos

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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