Merck
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C3155

Sigma-Aldrich

Catalase from bovine liver

aqueous solution, ≥30,000 units/mg protein

Synonym(s):
H2O2:H2O2 oxidoreductase
CAS Number:
Enzyme Commission number:
Número MDL:
NACRES:
NA.32

fonte biológica

bovine liver

Nível de qualidade

200

descrição

optimum pH ~ 7.0

esterilidade

aseptically filled

forma

aqueous solution

specific activity

≥30,000 units/mg protein

peso molecular

tetramer ~250 kDa

contém

≤0.10 mg/mL Thymol

ponto isoelétrico

5.4

pI 

5.4

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

2-8°C

Gene Information

cow ... CAT(280743)

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Aplicação

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Ações bioquímicas/fisiológicas

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.
This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Componentes

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Atenção

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Definição da unidade

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Nota de preparo

This product is an aqueous solution containing >30,000 units/mg protein. To remove the thymol preservative, the catalase crystals may be pelleted by centrifugation, the supernatant, discarded, the pellet resuspended in water, and then pelleted again. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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