Merck
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C3389

Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type VI-S, lyophilized powder, 200-1,000 units/mg protein

Synonym(s):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:

tipo

Type VI-S

Nível de qualidade

200

forma

lyophilized powder

specific activity

200-1,000 units/mg protein

peso molecular

280 kDa

composição

Protein, ≥60% biuret

solubilidade

20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)

application(s)

diagnostic assay manufacturing

temperatura de armazenamento

−20°C

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Descrição geral

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Aplicação

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Embalagem

500, 2000 units in glass bottle
10000 units in poly bottle

Ações bioquímicas/fisiológicas

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Definição da unidade

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

forma física

Lyophilized powder containing Tris buffer salts

Nota de preparo

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

Nota de análise

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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