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Catalase from Aspergillus niger

ammonium sulfate suspension, ≥4,000 units/mg protein

H2O2:H2O2 oxidoreductase
Número CAS:
Número da licença da enzima:
Número MDL:

Nível de qualidade



ammonium sulfate suspension

specific activity

≥4,000 units/mg protein

peso molecular

tetramer ~250 kDa

enviado em

wet ice

temperatura de armazenamento



Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.


10, 25 mg in glass bottle

Ações bioquímicas/fisiológicas

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.


Freezing of solutions is not recommended.

Definição da unidade

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

forma física

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Nota de análise

Protein determined by biuret

Código de classe de armazenamento

12 - Non Combustible Liquids



Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

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Certificado de origem

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Mais documentos

Quotes and Ordering

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Journal of bacteriology, 195(7), 1492-1503 (2013-01-22)
Spores of Clostridium difficile play a key role in the dissemination of this important human pathogen, and until recently little has been known of their functional characteristics. Genes encoding six spore coat proteins (cotA, cotB, cotCB, cotD, cotE, and sodA)
Nicolas Olivier et al.
PloS one, 8(7), e69004-e69004 (2013-07-23)
Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolutions down to a few tens of nanometers by exploiting the cycling of
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How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions


Enzymatic Assay of Catalase (EC

This procedure may be used for all Catalase products.

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