Merck
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C3515

Sigma-Aldrich

Catalase from Aspergillus niger

ammonium sulfate suspension, ≥4,000 units/mg protein

Sinônimo(s):
H2O2:H2O2 oxidoreductase
Número CAS:
Número da licença da enzima:
Número MDL:
eCl@ss:
32160410
NACRES:
NA.54

Nível de qualidade

200

forma

ammonium sulfate suspension

specific activity

≥4,000 units/mg protein

peso molecular

tetramer ~250 kDa

enviado em

wet ice

temperatura de armazenamento

2-8°C

Aplicação

Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.

Embalagem

10, 25 mg in glass bottle

Ações bioquímicas/fisiológicas

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Atenção

Freezing of solutions is not recommended.

Definição da unidade

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

forma física

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Nota de análise

Protein determined by biuret

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Mais documentos

Quotes and Ordering

Jacek Switala et al.
Archives of biochemistry and biophysics, 401(2), 145-154 (2002-06-11)
Catalases from 16 different organisms including representatives from all three phylogenetic clades were purified and characterized to provide a comparative picture of their respective properties. Collectively the enzymes presented a diverse range of activities and properties. Specific activities ranged from
Alejo Mosqueira et al.
Journal of neurochemistry, 152(6), 663-674 (2019-11-30)
Synaptic strength depends on the number of cell-surface neurotransmitter receptors in dynamic equilibrium with intracellular pools. Dysregulation of this homeostatic balance occurs, for example in myasthenia gravis, an autoimmune disease characterized by a decrease in the number of postsynaptic nicotinic
Patima Permpoonpattana et al.
Journal of bacteriology, 195(7), 1492-1503 (2013-01-22)
Spores of Clostridium difficile play a key role in the dissemination of this important human pathogen, and until recently little has been known of their functional characteristics. Genes encoding six spore coat proteins (cotA, cotB, cotCB, cotD, cotE, and sodA)
Nicolas Olivier et al.
PloS one, 8(7), e69004-e69004 (2013-07-23)
Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolutions down to a few tens of nanometers by exploiting the cycling of
Lydia Farack et al.
STAR protocols, 1(1), 100007-100007 (2020-10-29)
We describe an optimized smFISH protocol for the intact pancreas. The protocol is adapted from Lyubimova et al. (2013), a generic tissue smFISH protocol that works for most tissues but not the pancreas. The main changes implemented include increasing the period

Artigos

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolos

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

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