Merck
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C3556

Sigma-Aldrich

Catalase from human erythrocytes

≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

Synonym(s):
H2O2:H2O2 oxidoreductase
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:
NACRES:
NA.54

Nível de qualidade

200

teor

≥90% (SDS-PAGE)

forma

buffered aqueous solution

specific activity

≥30,000 units/mg protein

peso molecular

tetramer ~250 kDa

concentração

≤10 mg/mL

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

−20°C

Gene Information

human ... CAT(847)

Ações bioquímicas/fisiológicas

Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.
Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.

Definição da unidade

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

forma física

Solution in 50 mM Tris, pH 8.0

Nota de análise

Protein determined by biuret

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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