Merck
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C40

Sigma-Aldrich

Catalase from bovine liver

lyophilized powder, ≥10,000 units/mg protein

Synonym(s):
H2O2:H2O2 oxidoreductase
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:
eCl@ss:
32160410
NACRES:
NA.54

forma

lyophilized powder

Nível de qualidade

400

specific activity

≥10,000 units/mg protein

peso molecular

tetramer ~250 kDa

contém

≤0.2 wt. % Thymol

ponto isoelétrico

5.4

solubilidade

50 mM potassium phosphate buffer, pH 7.0: soluble 2.0 mg/mL

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

−20°C

Gene Information

cow ... CAT(280743)

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Embalagem

100, 500 mg in poly bottle
1 g in poly bottle

Aplicação

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Ações bioquímicas/fisiológicas

Catalase activity is constant over the pH range of 4.0-8.5. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.
Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

Componentes

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Atenção

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Definição da unidade

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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