Merck
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C5275

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture

Synonym(s):
Con A
CAS Number:
Número EC:
Número MDL:

esterilidade

aseptically processed

Nível de qualidade

200

tipo

Type IV-S

linha de produto

BioReagent

forma

lyophilized powder

technique(s)

cell culture | mammalian: suitable

Impurezas

salt (Contains buffer salts and NaCl)

solubilidade

PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy

temperatura de armazenamento

−20°C

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Descrição geral

Concanavalin A is a protein containing only β-sheets in its structure, and belongs to the legume lectins family.

Aplicação

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • in in vitro cell proliferation assay and cell cycle assessment
  • in lymphocyte proliferation
  • in splenocyte culturing

Ações bioquímicas/fisiológicas

Concanavalin A is known to stimulate acute hepatic inflammation and acts as a T‐cell mitogen. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy.
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Nota de análise

A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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